Human IL-15 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.91 pg/ml (50 μl) ； 4.20 pg/ml (10 μl) |
| Standard Curve Range | 15.63~1000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0569 | 0.0510 | 0.0540 | |
| 15.63 | 0.0986 | 0.0931 | 0.0959 | 0.0419 |
| 31.25 | 0.1385 | 0.1383 | 0.1384 | 0.0845 |
| 62.50 | 0.2296 | 0.2298 | 0.2297 | 0.1758 |
| 125.00 | 0.4239 | 0.4303 | 0.4271 | 0.3732 |
| 250.00 | 0.8343 | 0.8272 | 0.8308 | 0.7768 |
| 500.00 | 1.5410 | 1.4950 | 1.5180 | 1.4641 |
| 1000.00 | 2.8480 | 2.7570 | 2.8025 | 2.7486 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 20.0 | 97.8 | 305.8 | 25.0 | 120.8 | 332.4 |
| Standard Deviation | 1.0 | 7.6 | 14.0 | 1.8 | 9.4 | 17.0 |
| Coefficient of Variation(%) | 4.2 | 4.6 | 4.9 | 4.8 | 4.7 | 5.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-15 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 90% to 120% with an overall mean recovery of 109%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| serum | 30 | n.d.-14.63 | 6.7 | 14.63 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-15 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: IL-15
IL-15 (Interleukin 15) is a cytokine that induces or enhances the differentiation, maintenance, or activation of multiple T cell subsets including NK, NKT, Th17, Treg, and CD8+ memory cells. It also induces dendritic cell differentiation and inflammatory activation, exhibits anti-tumor activity, and inhibits the deposition of lipid in adipocytes. Complexes of IL-15 with cell surface IL-15 R alpha interact with complexes of IL-2 R beta and the Common gamma Chain on adjacent cells. This transpresentation mechanism enables cells to respond to IL-15 even if they do not express IL-15 R alpha. Ligation of membrane-associated IL-15/IL-15 R alpha complexes also induces reverse signaling that promotes activation of the IL-15/IL-15 R alpha expressing cells. The activity of circulating IL-15 is limited by its association with soluble IL-15 R alpha.
