Human IL-13R alpha 2 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 3.50 pg/ml (50 μl)；22.29 pg/ml (10 μl)； |
| Standard Curve Range | 27.43~20000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0106 | 0.0100 | 0.0103 | |
| 27.43 | 0.0238 | 0.0228 | 0.0233 | 0.0130 |
| 82.30 | 0.0507 | 0.0475 | 0.0491 | 0.0388 |
| 246.91 | 0.1314 | 0.1236 | 0.1275 | 0.1172 |
| 740.74 | 0.3798 | 0.3780 | 0.3789 | 0.3686 |
| 2222.22 | 1.0320 | 1.0130 | 1.0225 | 1.0122 |
| 6666.67 | 2.5480 | 2.5080 | 2.5280 | 2.5177 |
| 20000.00 | 4.1779 | 4.1531 | 4.1655 | 4.1552 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 372.5 | 1840.8 | 6053.0 | 379.6 | 1922.9 | 6355.7 |
| Standard Deviation | 20.6 | 126.5 | 290.5 | 18.2 | 110.3 | 206.8 |
| Coefficient of Variation(%) | 5.5 | 6.9 | 4.8 | 4.8 | 5.7 | 3.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-13 R alpha 2 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 105% with an overall mean recovery of 87%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-23.65 | 60 | 8.9 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-13 R alpha 2 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: IL-13R alpha 2
Two members of the type 5 subfamily of type I cytokine receptors can serve as receptors for IL-13. IL-13 can bind to IL-13 R alpha 1 (CD213a1; previously designated IL-13 R alpha or NR4) with low affinity, then recruits the IL-4 R alpha chain to form a high affinity receptor, causing downstream STAT6 activation. Alternately, IL-13 can bind IL-13 R alpha 2 (CD213a2) with high affinity; this interaction does not cause activation of STAT6, but does induce TGF-beta production. IL-13 R alpha 1 and IL-13 R alpha 2 each have three extracellular fibronectin type III domains, two cytokine receptor homology modules and a WSXWS motif typical of the class I cytokine receptor family, but IL-13 R alpha 2 has a much shorter cytoplasmic tail. IL-13 R subunits can be expressed on monocytes, macrophages, fibroblasts, human B cells, basophils, eosinophils, endothelial cells, and smooth muscle cells.
