Human CKMB enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.005 pg/ml (50 μl)；0.07 ng/ml (10 μl) |
| Standard Curve Range | 0.04~30 ng/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Ready-to-Use |
| Operation Duration | 120min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0144 | 0.0158 | 0.0151 | |
| 0.04 | 0.0332 | 0.0309 | 0.0321 | 0.0170 |
| 0.12 | 0.0716 | 0.0726 | 0.0721 | 0.0570 |
| 0.37 | 0.1778 | 0.1780 | 0.1779 | 0.1628 |
| 1.11 | 0.4794 | 0.4464 | 0.4629 | 0.4478 |
| 3.33 | 1.3260 | 1.3850 | 1.3555 | 1.3404 |
| 10.00 | 3.2180 | 3.2230 | 3.2205 | 3.2054 |
| 30.00 | 4.5063 | 4.4600 | 4.4832 | 4.4681 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 0.7 | 3.8 | 13.1 | 0.6 | 3.2 | 11.0 |
| Standard Deviation | 0.1 | 0.2 | 1.0 | 0.1 | 0.1 | 0.3 |
| Coefficient of Variation(%) | 5.6 | 4.6 | 7.3 | 3.6 | 3.9 | 3.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CKMB into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 79% to 119% with an overall mean recovery of 103%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-0.06 | 40 | 0.02 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CKMB in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: CKMB
The creatine kinase molecule is a dimer composed of a brain-type subunit (B) and a muscle-type subunit (M). There are usually three isozymes in normal human tissues, which are CK-BB (CK1), CK-MB (CK2) and CK-MM (CK3) in the order of electrophoresis. CK-BB is mainly found in smooth muscle and brain tissue, and almost none in serum; CK-MB mainly exists in myocardial tissue, accounting for 0~4% of CK in serum; CK-MM is mainly present in skeletal muscle, accounting for 96%~100% of CK in serum. CK-MB has its own subtypes, including MB2 (tissue form) and MB1 (transformed form). After the release of CK-MB2 from the myocardium, the carboxy-terminus is hydrolyzed by plasma carboxypeptidase N, and a lysine residue is removed to be converted to the serum-modified isoform CK-MB1. CK and CK-MB are one of the sensitive indicators of myocardial tissue injury, especially CK-MB is more specific. The increase in CK during myocardial injury is mainly due to the increase in CK-MB, which is manifested by the simultaneous increase of both.
