Human IL-10RB enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.17 pg/ml (50 μl)；1.65 pg/ml (10 μl)； |
| Standard Curve Range | 2.74~2000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0114 | 0.0103 | 0.0109 | |
| 2.74 | 0.0304 | 0.0268 | 0.0286 | 0.0178 |
| 8.23 | 0.0668 | 0.0605 | 0.0637 | 0.0528 |
| 24.69 | 0.1729 | 0.1688 | 0.1709 | 0.1600 |
| 74.07 | 0.4923 | 0.4784 | 0.4854 | 0.4745 |
| 222.22 | 1.3010 | 1.3120 | 1.3065 | 1.2957 |
| 666.67 | 3.0620 | 3.0470 | 3.0545 | 3.0437 |
| 2000.00 | 4.3703 | 4.3789 | 4.3746 | 4.3638 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 46.3 | 222.3 | 675.7 | 43.3 | 211.1 | 629.1 |
| Standard Deviation | 2.2 | 11 | 37.8 | 1.7 | 11.5 | 30.0 |
| Coefficient of Variation(%) | 4.7 | 5 | 5.6 | 3.8 | 5.4 | 4.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-10RB into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 95% to 126% with an overall mean recovery of 112%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 446.84-786.15 | 100 | 546.67 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: IL-10RB
IL-10 R beta, also known as IL-10 R2, mediates its biological activities via binding to a heteromeric receptor complex consisting of two distinct type II cytokine receptor subunits, the ligand binding IL-10 R alpha and the IL-10 R beta which does not bind IL-10 by itself but is required for signal transduction. The cDNA for human IL-10 R beta encodes a 325 amino acid (aa) type I transmembrane precursor protein with a 20 aa signal sequence, a 200 aa extracellular region, a 29 aa transmembrane segment and a 76 aa cytoplasmic domain. Within the extracellular region, there are two 100 aa subdomains that resemble the constant region of immunoglobulins. This structural motif is responsible for the alternative designation of IL-10 R beta as CRF2-4 (the 4th member of the cytokine receptor family class II/2). Human and mouse IL-10 R beta share approximately 69% aa sequence identity. Binding of the non-covalent IL-10 dimer to two IL-10 R alpha chains recruits two IL‑10 R beta chains resulting in the activation and phosphorylation of the signaling cascade involving JAK1, TYK2, and STAT3. IL-10 R beta is expressed ubiquitously. IL‑10 R beta is also a component of the IL-22 receptor complex consisting of the IL-10 R beta chain and IL-22 R, another type II cytokine receptor family member.
