Human IFN-gamma R1 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 2.28 pg/ml (50 μl)；11.4 pg/ml (10 μl) |
| Standard Curve Range | 13.72~10000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0285 | 0.0286 | 0.0286 | |
| 13.72 | 0.0405 | 0.0381 | 0.0393 | 0.0108 |
| 41.15 | 0.0631 | 0.0567 | 0.0599 | 0.0314 |
| 123.46 | 0.1253 | 0.1176 | 0.1215 | 0.0929 |
| 370.37 | 0.3195 | 0.2907 | 0.3051 | 0.2766 |
| 1111.11 | 0.8572 | 0.7749 | 0.8161 | 0.7875 |
| 3333.33 | 2.1650 | 2.0410 | 2.1030 | 2.0745 |
| 10000.00 | 4.0214 | 3.8750 | 3.9482 | 3.9197 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 216.4 | 1044.3 | 3466.9 | 188.9 | 972.7 | 3239.0 |
| Standard Deviation | 9.7 | 50.3 | 148.6 | 15.9 | 64.1 | 77.7 |
| Coefficient of Variation(%) | 4.5 | 4.8 | 4.3 | 4.4 | 6.6 | 2.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IFN gamma R1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 93% to 120% with an overall mean recovery of 103%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 284.50-993.11 | 100 | 576.85 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of VE Cadherin in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: IFN-gamma R1
The high-affinity IFN-gamma receptor complex is made up of two type I membrane proteins, IFN-gamma R1 (IFN-gamma R alpha ) and IFN-gamma R2 (IFN-gamma R beta ). Both proteins are members of the type II cytokine receptor family and share approximately 52% amino acid sequence identity. IFN-gamma R1 is the ligand-binding subunit that is necessary and sufficient for IFN-gamma binding and receptor internalization. IFN-gamma R2 is required for IFN-gamma signaling but does not bind IFN-gamma by itself. Human IFN-gamma R1 cDNA encodes a 499 amino acid (aa) residue protein with a 17 aa signal peptide, a 228 aa extracellular domain, a 23 aa transmembrane domain, and a 221 aa intracellular domain. Human and mouse IFN-gamma R1 share 52% amino acid sequence identity and bind IFN-gamma in a species-specific manner. IFN-gamma R1 is constitutively expressed in most cell types. Soluble IFN-gamma R1 that binds IFN-gamma has been detected in biological fluids.
