Human GITR/TNFRSF18 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.07 pg/ml (50 μl)；1.20 pg/ml (10 μl) |
| Standard Curve Range | 5.49~4000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0145 | 0.0146 | 0.0146 | |
| 5.49 | 0.0260 | 0.0256 | 0.0258 | 0.0113 |
| 16.46 | 0.0492 | 0.0497 | 0.0495 | 0.0349 |
| 49.38 | 0.1206 | 0.1200 | 0.1203 | 0.1058 |
| 148.15 | 0.3372 | 0.3175 | 0.3274 | 0.3128 |
| 444.44 | 0.9332 | 0.9130 | 0.9231 | 0.9086 |
| 1333.33 | 2.4945 | 2.4639 | 2.4792 | 2.4647 |
| 4000.00 | 4.1397 | 4.2936 | 4.2167 | 4.2021 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 1410.0 | 535.3 | 111.0 | 1556.0 | 680.1 | 136.7 |
| Standard Deviation | 55.5 | 10.1 | 2.4 | 102.5 | 32.1 | 9.1 |
| Coefficient of Variation(%) | 3.9 | 1.9 | 2.2 | 6.6 | 4.7 | 6.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human GITR/TNFRSF18 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 84% to 111% with an overall mean recovery of 98%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-1.19 | 75 | 0.47 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of VE Cadherin in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: GITR/TNFRSF18
GITR (glucocorticoid-induced tumor necrosis factor receptor, also named AITR, activation-inducible TNF R family member), is a 228 amino acid (aa) type I transmembrane protein belonging to the TNF R family and has been designated TNFRSF18. The GITR cytoplasmic domain has striking homology with the cytoplasmic domain of 4-1BB and CD27. Human GITR shares 55% homology with murine GITR. GITR is expressed at low levels in peripheral blood T cells, bone marrow, thymus, spleen, and lymph nodes. In contrast to mouse GITR, expression of human GITR is not induced by treatment with dexamethasone, but is
up-regulated by antigen stimulation or by treatment with anti-CD3 plus anti-CD28, or PMA plus ionomycin. Human GITR ligand was identified from human umbilical vein endothelial cells and is a 177 aa polypeptide belonging to the TNF superfamily (TNFSF18). Ligation of GITR can activate NF-kappa B through TRAF2, and protect T cells from TCR activation-induced cell death. It has been proposed that GITR ligand and GITR may modulate T lymphocyte functions.
