Human GDF-15 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.11 pg/ml (10 μl) |
| Standard Curve Range | 4.10~1000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0080 | 0.0089 | 0.0085 | |
| 4.10 | 0.0208 | 0.0223 | 0.0216 | 0.0131 |
| 10.24 | 0.0417 | 0.0405 | 0.0411 | 0.0327 |
| 25.60 | 0.0956 | 0.0898 | 0.0927 | 0.0843 |
| 64.00 | 0.2179 | 0.2061 | 0.2120 | 0.2036 |
| 160.00 | 0.5291 | 0.4912 | 0.5102 | 0.5017 |
| 400.00 | 1.2370 | 1.1710 | 1.2040 | 1.1956 |
| 1000.00 | 2.5730 | 2.5450 | 2.5590 | 2.5506 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 19.6 | 97.1 | 299.2 | 20.6 | 98.6 | 311.8 |
| Standard Deviation | 1.1 | 3.5 | 14.6 | 0.8 | 2.5 | 8.4 |
| Coefficient of Variation(%) | 5.7 | 3.6 | 4.9 | 4.0 | 2.5 | 2.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human GDF-15 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 105% to 120% with an overall mean recovery of 117%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 61.09-290.23 | 100 | 132.88 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: GDF-15
GDF-15 (Growth Differentiation Factor 15) is a dimeric TGF-beta superfamily cytokine that is highly expressed in placenta and brain. It promotes neuronal survival and regulates neuropeptide Y and pro-opiomelanocortin levels to suppress appetite. It is cardioprotective via inhibition of platelet activation, limiting atherosclerosis, promoting recovery following myocardial infarction, and regulating angiogenesis. GDF-15 also inhibits cartilage formation and the production of TNF-alpha production from LPS-stimulated macrophages.
