Human FGF-21 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 3.97 pg/ml (50 μl)；27.21 pg/ml (10 μl)； |
| Standard Curve Range | 40.96~10000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120 min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0460 | 0.0428 | 0.0444 | |
| 40.96 | 0.0864 | 0.0780 | 0.0822 | 0.0378 |
| 102.40 | 0.1312 | 0.1391 | 0.1352 | 0.0908 |
| 256.00 | 0.2677 | 0.2552 | 0.2615 | 0.2171 |
| 640.00 | 0.5469 | 0.5421 | 0.5445 | 0.5001 |
| 1600.00 | 1.2130 | 1.1850 | 1.1990 | 1.1546 |
| 4000.00 | 2.4020 | 2.4170 | 2.4095 | 2.3651 |
| 10000.00 | 3.8410 | 3.8460 | 3.8435 | 3.7991 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 158.4 | 869.8 | 2834.0 | 157.6 | 862.8 | 2716.3 |
| Standard Deviation | 7.2 | 42.7 | 126.6 | 4.3 | 36.3 | 124.2 |
| Coefficient of Variation(%) | 4.6 | 4.9 | 4.5 | 2.7 | 4.2 | 4.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human FGF-21 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 87% to 120% with an overall mean recovery of 111%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| serum | 30 | 31.76-2828.07 | 100.0 | 864.79 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human FGF-21 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: FGF-21
FGF-21 (Fibroblast Growth Factor 21) is a member of an FGF subfamily that also includes FGF-19 and FGF-23. FGF-19 subfamily members lack a heparin binding domain, enabling them to freely circulate as endocrine factors and diffuse within tissues. FGF-21 signals through Klotho beta in complex with FGF receptors. It regulates cellular metabolism including glucose uptake in adipocytes and cellular sensitivity to insulin. It is basally expressed in the pancreas, thymus, liver, and adipose tissue and is upregulated in liver, muscle, and fat in obesity and diabetes. FGF-21 also plays a role in cell survival and proliferation, mesenchymal stem cell differentiation, circadian rhythm, and controlling reproductive capacity.
