Human FABP2 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 1.85 pg/ml (50 μl)；9.48 pg/ml (10 μl)； |
| Standard Curve Range | 10.97~8000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0127 | 0.0117 | 0.0122 | |
| 10.97 | 0.0221 | 0.0197 | 0.0209 | 0.0087 |
| 32.92 | 0.0360 | 0.0352 | 0.0356 | 0.0234 |
| 98.77 | 0.0823 | 0.0774 | 0.0799 | 0.0677 |
| 296.30 | 0.2234 | 0.2171 | 0.2203 | 0.2081 |
| 888.89 | 0.6406 | 0.6136 | 0.6271 | 0.6149 |
| 2666.67 | 1.7600 | 1.7220 | 1.7410 | 1.7288 |
| 8000.00 | 3.7530 | 3.8030 | 3.7780 | 3.7658 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 2676.88 | 824.36 | 163.87 | 2575.6 | 777.7 | 160.5 |
| Standard Deviation | 151.57 | 35.86 | 10.04 | 60.3 | 14.0 | 7.1 |
| Coefficient of Variation(%) | 5.7 | 4.4 | 6.1 | 2.3 | 2.3 | 3.2 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human FABP2 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 115% with an overall mean recovery of 101%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 517.21-2083.92 | 100 | 1262.00 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: FABP2
The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Intestinal fatty acid-binding protein 2 gene contains four exons and is an abundant cytosolic protein in small intestine epithelial cells. This gene has a polymorphism at codon 54 that identified an alanine-encoding allele and a threonine-encoding allele. Thr-54 protein is associated with increased fat oxidation and insulin resistance.
