Human CXCL13/BCA-1 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.56 pg/ml (50 μl)；5.32 pg/ml (10 μl)； |
| Standard Curve Range | 7.81~500 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0076 | 0.0074 | 0.0075 | |
| 7.81 | 0.0260 | 0.0265 | 0.0263 | 0.0188 |
| 15.63 | 0.0515 | 0.0509 | 0.0512 | 0.0437 |
| 31.25 | 0.1190 | 0.1180 | 0.1185 | 0.1110 |
| 62.50 | 0.2836 | 0.2700 | 0.2768 | 0.2693 |
| 125.00 | 0.7076 | 0.6990 | 0.7033 | 0.6958 |
| 250.00 | 1.8240 | 1.8140 | 1.8190 | 1.8115 |
| 500.00 | 3.5660 | 3.6350 | 3.6005 | 3.5930 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 19.3 | 78.3 | 213.9 | 18.5 | 76.1 | 216.7 |
| Standard Deviation | 0.7 | 2.7 | 9.6 | 0.7 | 2.3 | 7.1 |
| Coefficient of Variation(%) | 3.4 | 3.4 | 4.5 | 3.8 | 3.0 | 3.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CXCL13/BCA-1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 120% with an overall mean recovery of 96%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 28.44-275.19 | 100 | 71.8 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CXCL13/BCA-1 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: CXCL13/BCA-1
CXCL13/BLC/BCA-1 is a constitutively expressed chemokine that plays an important role in B and T cell homing. It is expressed by salivary gland epithelium, dendritic cells, osteoclasts, and peritoneal macrophages. It can form homodimers or heterodimers with FGF basic, and it signals through CXCR3 or CXCR5. CXCL13 induces the migration of naïve B cells and a subset of memory T cells to lymphoid tissue. It also promotes B1 cell migration into the omentum and peritoneum. In the fetus, CXCL13 attracts CD4+ CD3- IL-7 R alpha+ hematopoietic cells to sites of future Peyer’s patch development.
