Human/Monkey/Canine CXCL10/IP-10 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.16 pg/ml (50 μl)；0.68 pg/ml (10 μl) |
| Standard Curve Range | 2.74~2000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0150 | 0.0145 | 0.0148 | |
| 2.74 | 0.0382 | 0.0344 | 0.0363 | 0.0216 |
| 8.23 | 0.0861 | 0.0827 | 0.0844 | 0.0697 |
| 24.69 | 0.2315 | 0.2262 | 0.2289 | 0.2141 |
| 74.07 | 0.6512 | 0.6404 | 0.6458 | 0.6311 |
| 222.22 | 1.8430 | 1.6300 | 1.7365 | 1.7218 |
| 666.67 | 3.1870 | 3.1500 | 3.1685 | 3.1538 |
| 2000.00 | 3.9030 | 3.8160 | 3.8595 | 3.8448 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 52.3 | 262.9 | 708.7 | 42.8 | 226.4 | 619.0 |
| Standard Deviation | 3.2 | 12.6 | 39.7 | 1.4 | 9.9 | 10.4 |
| Coefficient of Variation(%) | 6.1 | 4.8 | 5.6 | 3.3 | 4.4 | 1.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CXCL10 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 117% with an overall mean recovery of 100%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Human Serum | 30 | 8.36-99.65 | 100 | 25.02 |
Serum/Plasma – Thirty samples from apparently healthy volunteers, two samples from healthy monkeys and five samples from healthy canines were evaluated for the presence of human CXCL10 in this assay. No medical histories were available for the donors.
n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: CXCL10/IP-10
IP-10 was originally identified as an IFN-gamma-inducible gene in monocytes, fibroblasts and endothelial cells. The mouse homolog of human IP-10, CRG-2, shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of IP-10 identified the protein as a member of the CXC chemokine subfamily.
