Human CXCL10/IP-10 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.16 pg/ml (50 μl)；0.68 pg/ml (10 μl) |
| Standard Curve Range | 2.74~2000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0150 | 0.0145 | 0.0148 | |
| 2.74 | 0.0382 | 0.0344 | 0.0363 | 0.0216 |
| 8.23 | 0.0861 | 0.0827 | 0.0844 | 0.0697 |
| 24.69 | 0.2315 | 0.2262 | 0.2289 | 0.2141 |
| 74.07 | 0.6512 | 0.6404 | 0.6458 | 0.6311 |
| 222.22 | 1.8430 | 1.6300 | 1.7365 | 1.7218 |
| 666.67 | 3.1870 | 3.1500 | 3.1685 | 3.1538 |
| 2000.00 | 3.9030 | 3.8160 | 3.8595 | 3.8448 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 52.3 | 262.9 | 708.7 | 42.8 | 226.4 | 619.0 |
| Standard Deviation | 3.2 | 12.6 | 39.7 | 1.4 | 9.9 | 10.4 |
| Coefficient of Variation(%) | 6.1 | 4.8 | 5.6 | 3.3 | 4.4 | 1.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CXCL10 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 117% with an overall mean recovery of 100%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 8.36-99.65 | 100 | 25.02 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CXCL10 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: CXCL10/IP-10
IP-10 was originally identified as an IFN-gamma-inducible gene in monocytes, fibroblasts and endothelial cells. The mouse homolog of human IP-10, CRG-2, shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of IP-10 identified the protein as a member of the CXC chemokine subfamily.
