Human CTGF/CCN2 enzyme-linked immunoassay kit(one step)
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.07 ng/ml (50 μl)；0.35 ng/ml (10 μl)； |
| Standard Curve Range | 4.69~300 ng/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 60min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0068 | 0.0064 | 0.0066 | |
| 4.69 | 0.0893 | 0.0926 | 0.0910 | 0.0844 |
| 9.38 | 0.2235 | 0.2204 | 0.2220 | 0.2154 |
| 18.75 | 0.4926 | 0.5205 | 0.5066 | 0.5000 |
| 37.50 | 1.0290 | 1.1290 | 1.0790 | 1.0724 |
| 75.00 | 1.9120 | 2.0470 | 1.9795 | 1.9729 |
| 150.00 | 2.9800 | 3.1140 | 3.0470 | 3.0404 |
| 300.00 | 3.5890 | 3.6480 | 3.6185 | 3.6119 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 6.3 | 31.3 | 98.9 | 6.0 | 31.1 | 101.0 |
| Standard Deviation | 0.2 | 1.0 | 2.8 | 0.2 | 1.3 | 4.1 |
| Coefficient of Variation(%) | 3.6 | 3.3 | 2.9 | 3.8 | 4.2 | 4.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CTGF/CCN2 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 88% to 105% with an overall mean recovery of 96%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 12.33-36.79 | 100 | 23.86 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CTGF/CCN2 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: CTGF/CCN2
CTGF belongs to the CCN (CYR61/CTGF/NOV) family of secreted proteins that share a common multimodular organization. Each protein contains an IGF-binding protein domain, a von Willebrand factor type C domain, a thrombospondin type I domain, and a cysteine knot domain. The multimodular CTGF has the ability to bind multiple ligands and has numerous effects on development, differentation, and disease. The C-terminal cysteine knot motif contains the heparin and low density lipoprotein receptor (LDLR) binding sites that contribute to the adhesive and mitogenic properties of CTGF.
