Human Complement factor D enzyme-linked immunoassay kit（one step）
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.01 ng/ml (10 μl) |
| Standard Curve Range | 0.41~300 ng/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | serum, plasma |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 60min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0089 | 0.0083 | 0.0086 | |
| 0.41 | 0.0171 | 0.0172 | 0.0172 | 0.0086 |
| 1.23 | 0.0395 | 0.0399 | 0.0397 | 0.0311 |
| 3.70 | 0.1106 | 0.1147 | 0.1127 | 0.1041 |
| 11.11 | 0.3221 | 0.3323 | 0.3272 | 0.3186 |
| 33.33 | 0.9434 | 0.9330 | 0.9382 | 0.9296 |
| 100.00 | 2.4900 | 2.4320 | 2.4610 | 2.4524 |
| 300.00 | 4.3547 | 4.3549 | 4.3548 | 4.3462 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 4.9 | 24.7 | 90.1 | 5.1 | 25.9 | 94.7 |
| Standard Deviation | 0.1 | 0.7 | 3.8 | 0.2 | 1.1 | 4.2 |
| Coefficient of Variation(%) | 2.1 | 2.7 | 4.2 | 4.1 | 4.1 | 4.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Complement factor D into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 92% to 105% with an overall mean recovery of 98%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 433.18-943.01 | 100 | 713.99 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: Complement factor D
Complement Factor D, also known as adipsin, is a serine protease that catalyzes the initial proteolytic step in the alternative pathway of complement. It is an exceptionally specific protease and the only known protein substrate is factor B in complex with C3.3 Factor D protease activity is regulated by reversible conformational changes, which differs from the majority of serine proteases whose regulation involves either activation by processing of the zymogens or inactivation by binding of the inhibitors.
