Human Complement factor B enzyme-linked immunoassay kit(one step)
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.62 ng/ml (10 μl) |
| Standard Curve Range | 46.88~3000 ng/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | serum, plasma |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 60 min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0687 | 0.0675 | 0.0681 | |
| 46.88 | 0.1696 | 0.1820 | 0.1758 | 0.1077 |
| 93.75 | 0.2560 | 0.2624 | 0.2592 | 0.1911 |
| 187.50 | 0.4569 | 0.4582 | 0.4576 | 0.3895 |
| 375.00 | 0.7900 | 0.7890 | 0.7895 | 0.7214 |
| 750.00 | 1.4130 | 1.4390 | 1.4260 | 1.3579 |
| 1500.00 | 2.2060 | 2.2270 | 2.2165 | 2.1484 |
| 3000.00 | 2.8310 | 2.9550 | 2.8930 | 2.8249 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 85.84 | 349.12 | 1022.87 | 86.70 | 347.83 | 981.61 |
| Standard Deviation | 2.58 | 9.23 | 41.12 | 2.40 | 10.45 | 29.08 |
| Coefficient of Variation(%) | 3.0 | 2.6 | 4.0 | 2.8 | 3.0 | 3.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Human Complement factor B into health Human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 119% with an overall mean recovery of 105%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 83.49-233.23 | 100 | 151.46 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Complement factor B in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: Complement factor B
Complement factor B is a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease that associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction. The polyadenylation site of this gene is 421 bp from the 5' end of the gene for complement component 2.
