Human CNTF enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.25 pg/ml (50 μl)；3.77 pg/ml (10 μl) |
| Standard Curve Range | 2.74~2000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0328 | 0.0323 | 0.0326 | |
| 2.74 | 0.0495 | 0.0492 | 0.0494 | 0.0168 |
| 8.23 | 0.0814 | 0.0796 | 0.0805 | 0.0480 |
| 24.69 | 0.1741 | 0.1868 | 0.1805 | 0.1479 |
| 74.07 | 0.4648 | 0.4985 | 0.4817 | 0.4491 |
| 222.22 | 1.2410 | 1.3150 | 1.2780 | 1.2455 |
| 666.67 | 3.0640 | 3.2330 | 3.1485 | 3.1160 |
| 2000.00 | 4.5481 | 4.6198 | 4.5840 | 4.5514 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 39.2 | 197.9 | 618.1 | 37.0 | 199.0 | 601.7 |
| Standard Deviation | 1.7 | 8.3 | 30.7 | 2.2 | 14.8 | 39.7 |
| Coefficient of Variation(%) | 4.4 | 4.2 | 5.0 | 6.0 | 7.4 | 6.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CNTF into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 74% to 112% with an overall mean recovery of 96%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-81.17 | 13 | 42.58 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CNTF in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: CNTF
Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons and hippocampal neurons. CNTF has also been shown to prevent the degeneration of motor axons after axotomy.
The gene for human CNTF has been localized to the proximal region of the long arm of chromosome 11. The cDNA for human CNTF encodes a 200 amino acid residue polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL-6, IL-11, LIF and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes.
