Human CHIT1 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.22 pg/ml (50 μl)；0.49 pg/ml (10 μl)； |
| Standard Curve Range | 2.74~2000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0155 | 0.0136 | 0.0146 | |
| 2.74 | 0.0232 | 0.0218 | 0.0225 | 0.0080 |
| 8.23 | 0.0381 | 0.0399 | 0.0390 | 0.0245 |
| 24.69 | 0.1039 | 0.0986 | 0.1013 | 0.0867 |
| 74.07 | 0.2901 | 0.3065 | 0.2983 | 0.2838 |
| 222.22 | 0.8336 | 0.9318 | 0.8827 | 0.8682 |
| 666.67 | 2.3258 | 2.3320 | 2.3289 | 2.3144 |
| 2000.00 | 4.0844 | 4.0230 | 4.0537 | 4.0392 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 47.01 | 199.00 | 780.94 | 46.20 | 201.75 | 671.27 |
| Standard Deviation | 1.72 | 9.54 | 45.28 | 1.93 | 5.46 | 38.00 |
| Coefficient of Variation(%) | 3.7 | 4.8 | 5.8 | 4.2 | 2.7 | 5.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CHIT1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 83% to 115% with an overall mean recovery of 97%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 0.24-11.50 | 100 | 4.04 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CHIT1 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: CHIT1
Chitotriosidase encoded by the CHIT1 gene is a member of the chitinase family that is selectively expressed in activated tissue macrophages. It is distinct from another member, known as acidic mammalian chitinase (AMC) encoded by the CHIA gene and expressed mainly in the gastrointestinal tract and lung. Both CHIA and CHIT1 are secreted as 50 kDa proteins. In contrast to CHIA, CHIT1 is not stable under acidic pHs and can be processed into a C-terminally truncated 39 kDa form. CHIT1 is the best biomarker in the monitoring of Gaucher disease among the three most commonly used markers that also include acid phosphatase and angiotensin-converting enzyme (ACE). CHIT1 is also a specific marker of macrophage activation in acute ischemic stroke.
