Human CD62E enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 1.02 pg/ml (50 μl)；5.13 pg/ml (10 μl)； |
| Standard Curve Range | 8.23~6000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0179 | 0.0184 | 0.0182 | |
| 8.23 | 0.0352 | 0.0343 | 0.0348 | 0.0166 |
| 24.69 | 0.0677 | 0.0684 | 0.0681 | 0.0499 |
| 74.07 | 0.1695 | 0.1696 | 0.1696 | 0.1514 |
| 222.22 | 0.4737 | 0.4691 | 0.4714 | 0.4533 |
| 666.67 | 1.2900 | 1.3210 | 1.3055 | 1.2874 |
| 2000.00 | 3.1610 | 3.2220 | 3.1915 | 3.1734 |
| 6000.00 | 4.4674 | 4.4727 | 4.4701 | 4.4519 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 163.0 | 726.7 | 2062.0 | 124.4 | 594.9 | 1727.9 |
| Standard Deviation | 9.2 | 29.1 | 128.8 | 5.2 | 31.9 | 74.36 |
| Coefficient of Variation(%) | 5.6 | 4.0 | 6.2 | 4.2 | 5.4 | 4.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CD62E into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 85% to 115% with an overall mean recovery of 105%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 8.16-43.12 | 100 | 19.69 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CD62E in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: CD62E
E-Selectin, also known as Endothelial Leukocyte Adhesion Molecule 1 (ELAM-1), and Leukocyte-Endothelial Cell Adhesion Molecule 2 (LECAM-2), has been designated CD62E. It is a cell-surface glycoprotein expressed by activated endothelium and it mediates the adhesion of blood neutrophils.
