Human CD200R1 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.1 pg/ml (50 μl)；3.09 pg/ml (10 μl) |
| Standard Curve Range | 6.86~5000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0140 | 0.0153 | 0.0147 | |
| 6.86 | 0.0195 | 0.0207 | 0.0201 | 0.0055 |
| 20.58 | 0.0312 | 0.0276 | 0.0294 | 0.0148 |
| 61.73 | 0.0620 | 0.0644 | 0.0632 | 0.0486 |
| 185.19 | 0.1621 | 0.1541 | 0.1581 | 0.1435 |
| 555.56 | 0.4406 | 0.4431 | 0.4419 | 0.4272 |
| 1666.67 | 1.2060 | 1.2180 | 1.2120 | 1.1974 |
| 5000.00 | 2.7690 | 2.6630 | 2.7160 | 2.7014 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 1690.5 | 461.7 | 84.6 | 1620.9 | 435.3 | 81.4 |
| Standard Deviation | 121.9 | 24.2 | 4.1 | 109.3 | 27.2 | 5.5 |
| Coefficient of Variation(%) | 7.2 | 5.2 | 4.8 | 6.7 | 6.2 | 6.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CD200R1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 76% to 123% with an overall mean recovery of 102%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 747.49-1789.41 | 100 | 1147.83 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-12/IL-23 p40 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: CD200R1
CD200 R1, also known as OX-2 receptor, is a 90 kDa, type I transmembrane protein that belongs to the immunoglobulin superfamily. CD200 R1 is important in the regulation of myeloid cell activity. The mouse CD200 R1 cDNA encodes a 326 aa precursor that includes a 25 aa signal sequence, a 213 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 67 aa cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain. Within the ECD, mouse CD200 R1 shares 56% and 70% aa sequence identity with human and rat CD200 R1, respectively. The ECD of mouse CD200 R1 shares 69%, 38%, 79%, and 83% aa sequence identity with the ECD of CD200 R2, 3, 4, and a CD200 R-like molecule, respectively. CD200 R1 is expressed primarily on mast cells, basophils, macrophages, and dendritic cells, while its ligand, CD200, is widely distributed. Disruption of this receptor-ligand pair by knockout of the CD200 gene leads to increased macrophage number and activation, plus a predisposition to autoimmune disorders. Association of CD200 with CD200 R1 takes place between their respective N-terminal Ig-like domains. The CD200 R-like molecules may interact differently with CD200. The cytoplasmic domain of CD200 R1 contains two non-ITIM tyrosine residues which are required for propagating its inhibitory signals. CD200 R-like molecules, in contrast, are potentially activating receptors by means of their association with DAP12.
