Human CCL20/MIP-3 alpha enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.72 pg/ml (50 μl) ； 0.89 pg/ml (10 μl) |
| Standard Curve Range | 4.12~3000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0096 | 0.0096 | 0.0096 | |
| 4.12 | 0.0226 | 0.0239 | 0.0233 | 0.0137 |
| 12.35 | 0.0538 | 0.0582 | 0.0560 | 0.0464 |
| 37.04 | 0.1590 | 0.1586 | 0.1588 | 0.1492 |
| 111.11 | 0.4436 | 0.4379 | 0.4408 | 0.4312 |
| 333.33 | 1.1440 | 1.2350 | 1.1895 | 1.1799 |
| 1000.00 | 2.4690 | 2.4030 | 2.4360 | 2.4264 |
| 3000.00 | 3.6820 | 3.7510 | 3.7165 | 3.7069 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 67.2 | 320.5 | 1005.9 | 58.9 | 310.9 | 1026.1 |
| Standard Deviation | 3.1 | 15.5 | 45.6 | 2.2 | 11.8 | 40.1 |
| Coefficient of Variation(%) | 4.6 | 4.8 | 4.5 | 3.7 | 3.8 | 3.9 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CCL20/MIP-3 alpha into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 119% with an overall mean recovery of 103%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 4.01-2566.88 | 100 | 606.71 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CCL20 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: CCL20/MIP-3 alpha
CCL20, also known as MIP-3 alpha, is a chemokine that plays an important role in dendritic cell trafficking and recruitment and activation of T cells. It is upregulated in monocytes, T cells, endothelial cells, epithelial cells, and fibroblasts following inflammatory stimulation. CCL20 signals through CCR6 which is expressed on memory T cells, B cells and bone marrow-derived dendritic cells.
