Human Caspase-9 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.41 pg/ml (50 μl)；4.11 pg/ml (10 μl)； |
| Standard Curve Range | 13.72~10000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120 min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0069 | 0.0065 | 0.0067 | |
| 13.72 | 0.0281 | 0.0273 | 0.0277 | 0.0210 |
| 41.15 | 0.0736 | 0.0769 | 0.0753 | 0.0686 |
| 123.46 | 0.2208 | 0.2160 | 0.2184 | 0.2117 |
| 370.37 | 0.6230 | 0.6025 | 0.6128 | 0.6061 |
| 1111.11 | 1.5260 | 1.4650 | 1.4955 | 1.4888 |
| 3333.33 | 2.8990 | 2.9790 | 2.9390 | 2.9323 |
| 10000.00 | 3.9550 | 4.0994 | 4.0272 | 4.0205 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 154.1 | 1139.3 | 2527.1 | 184.8 | 1179.4 | 3612.7 |
| Standard Deviation | 7.2 | 53.5 | 188.9 | 10.2 | 31.3 | 50.6 |
| Coefficient of Variation(%) | 4.7 | 4.7 | 7.5 | 5.5 | 2.7 | 1.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Caspase-9 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 89% to 111% with an overall mean recovery of 93%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 359.91-4346.98 | 100 | 1798.80 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Caspase-9 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: Caspase-9
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNF-alpha, TRAIL, anti-CD-95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues.
