Human Angiotensinogen enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.25 ng/ml (10 μl) |
| Standard Curve Range | 4.69~300 ng/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120 min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0403 | 0.0513 | 0.0458 | |
| 4.69 | 0.0701 | 0.0763 | 0.0732 | 0.0274 |
| 9.38 | 0.1047 | 0.1146 | 0.1097 | 0.0639 |
| 18.75 | 0.1803 | 0.1798 | 0.1801 | 0.1343 |
| 37.50 | 0.3458 | 0.3212 | 0.3335 | 0.2877 |
| 75.00 | 0.6617 | 0.6878 | 0.6748 | 0.6290 |
| 150.00 | 1.3420 | 1.1830 | 1.2625 | 1.2167 |
| 300.00 | 2.5350 | 2.7620 | 2.6485 | 2.6027 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 6.24 | 31.12 | 96.08 | 6.93 | 30.70 | 97.07 |
| Standard Deviation | 0.44 | 1.54 | 7.09 | 0.49 | 1.67 | 3.79 |
| Coefficient of Variation(%) | 7.1 | 5.0 | 7.4 | 7.1 | 5.4 | 3.9 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Angiotensinogen into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 86% to 120% with an overall mean recovery of 108%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 29.69-62.05 | 100.0 | 46.34 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Angiotensinogen in this assay. No medical histories were available for the donors.
n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: Angiotensinogen
Serpin A8 (serine proteinase inhibitor-clade A #8; also angiotensinogen/ANGT) is a secreted, 52-62 kDa glycoprotein member of the clade F-subfamily, serpin superfamily of protease inhibitors. It is expressed by neurons and hepatocytes, and undergoes extracellular cleavage by renin to create a ten amino acid (aa) peptide termed Ang/angiotensin I. This inactive peptide is further cleaved by ACE on the endothelial cell membrane to create bioactive Ang II. Ang II induces vascoconstriction and aldosterone release. Mature human angiotensinogen is 452 aa in length (aa 34-485). It contains Ang I (aa 34-43) that is cleaved to create Ang II (aa 34-41). Angiotensinogen may circulate in a 200 kDa complex with major basic protein (MBP), or as part of a larger 300 kDa complex with MBP and complement C3dg. Over aa 34-485, human serpin A8 shares 61% aa identity with mouse serpin A8.
