Human Angiogenin enzyme-linked immunoassay kit（one step）
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.01 ng/ml (50 μl)；0.07 ng/ml (10 μl)； |
| Standard Curve Range | 0.41~100 ng/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 60min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0123 | 0.0123 | 0.0123 | |
| 0.41 | 0.0423 | 0.0436 | 0.0430 | 0.0307 |
| 1.02 | 0.0915 | 0.0921 | 0.0918 | 0.0795 |
| 2.56 | 0.2093 | 0.2128 | 0.2111 | 0.1988 |
| 6.40 | 0.5093 | 0.5195 | 0.5144 | 0.5021 |
| 16.00 | 1.2320 | 1.2210 | 1.2265 | 1.2142 |
| 40.00 | 2.5030 | 2.4640 | 2.4835 | 2.4712 |
| 100.00 | 3.4960 | 3.4790 | 3.4875 | 3.4752 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 2.2 | 9.9 | 34.2 | 2.4 | 10.6 | 35.4 |
| Standard Deviation | 0.1 | 0.1 | 0.6 | 0.0 | 0.2 | 0.6 |
| Coefficient of Variation(%) | 2.3 | 1.4 | 1.6 | 1.1 | 1.7 | 1.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Angiogenin into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 119% with an overall mean recovery of 96%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 115.46-277.38 | 100 | 168.44 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Angiogenin in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: Angiogenin
Angiogenin (ANG) is a secreted protein that belongs to the pancreatic ribonuclease family. It was initially purified based on its ability to initiate vascularization in the chicken embryo chorioallantoic membrane from serum-free media conditioned by growth of a human adenocarcinoma cell line HT-29. A number of other tumors, as well as normal cell lines, also secrete Angiogenin. Angiogenin is present in normal human plasma.
