Human Alpha-Synuclein enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.30 ng/ml (50 μl)；0.60 ng/ml (10 μl)； |
| Standard Curve Range | 0.78~50 ng/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0108 | 0.0105 | 0.0107 | |
| 0.78 | 0.0171 | 0.0166 | 0.0169 | 0.0062 |
| 1.56 | 0.0328 | 0.0294 | 0.0311 | 0.0205 |
| 3.13 | 0.0793 | 0.0817 | 0.0805 | 0.0699 |
| 6.25 | 0.2713 | 0.2822 | 0.2768 | 0.2661 |
| 12.50 | 1.0200 | 1.0220 | 1.0210 | 1.0104 |
| 25.00 | 3.0020 | 3.0060 | 3.0040 | 2.9934 |
| 50.00 | 4.5515 | 4.5446 | 4.5481 | 4.5374 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 3.1 | 8.6 | 22.3 | 2.7 | 7.8 | 20.3 |
| Standard Deviation | 0.1 | 0.2 | 0.9 | 0.1 | 0.2 | 0.5 |
| Coefficient of Variation(%) | 3.8 | 2.9 | 4.0 | 2.9 | 2.8 | 2.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Alpha-Synuclein into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 83% to 120% with an overall mean recovery of 99%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 126.53-645.86 | 100 | 269.28 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Alpha-Synuclein in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: Alpha-Synuclein
alpha -Synuclein (SNCA) is a cytoplasmic protein that is predominantly expressed in the central nervous system. It is a minor protein found in amyloid deposition that is a hallmark of Alzheimer's disease. Defects in SNCA are associated with familial Parkinson's disease.
