Human Adiponectin enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.15 pg/ml (50 μl)；3.56 pg/ml (10 μl) |
| Standard Curve Range | 6.86~5000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0097 | 0.0102 | 0.0100 | |
| 6.86 | 0.0277 | 0.0283 | 0.0280 | 0.0181 |
| 20.58 | 0.0665 | 0.0592 | 0.0629 | 0.0529 |
| 61.73 | 0.1897 | 0.1883 | 0.1890 | 0.1791 |
| 185.19 | 0.5615 | 0.5451 | 0.5533 | 0.5434 |
| 555.56 | 1.4160 | 1.4190 | 1.4175 | 1.4076 |
| 1666.67 | 2.8940 | 2.7930 | 2.8435 | 2.8336 |
| 5000.00 | 4.1026 | 4.1065 | 4.1046 | 4.0946 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 103.3 | 485.3 | 1632.6 | 111.5 | 520.8 | 1724.6 |
| Standard Deviation | 2.8 | 27.8 | 81.6 | 5.8 | 31.7 | 128.9 |
| Coefficient of Variation(%) | 2.7 | 5.7 | 5.0 | 5.2 | 6.1 | 7.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Adiponectin into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 126% with an overall mean recovery of 93%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 2621.9-7072.8 | 100 | 4559.1 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of VE Cadherin in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: Adiponectin
Adiponectin (also known as Adipocyte complement-related protein of 30 kDa, Acrp30) is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. Adiponectin associates into 70 kDa noncovalently-linked trimers that are disulfide-linked into medium molecular weight hexamers and then into >300 kDa high molecular weight oligomers.
