Human Acetylcholinesterase/ACHE enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.85 pg/ml (50 μl)；8.61 pg/ml (10 μl)； |
| Standard Curve Range | 40.96~10000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0099 | 0.0094 | 0.0097 | |
| 40.96 | 0.0568 | 0.0541 | 0.0555 | 0.0458 |
| 102.40 | 0.1282 | 0.1271 | 0.1277 | 0.1180 |
| 256.00 | 0.3135 | 0.2983 | 0.3059 | 0.2963 |
| 640.00 | 0.7399 | 0.6988 | 0.7194 | 0.7097 |
| 1600.00 | 1.6520 | 1.6190 | 1.6355 | 1.6259 |
| 4000.00 | 3.1140 | 2.9580 | 3.0360 | 3.0264 |
| 10000.00 | 4.3172 | 4.2587 | 4.2880 | 4.2783 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 174.5 | 844.4 | 2868.9 | 182.3 | 847.9 | 2927.1 |
| Standard Deviation | 3.7 | 23.5 | 108.7 | 9.0 | 40.8 | 78.6 |
| Coefficient of Variation(%) | 2.1 | 2.8 | 3.8 | 4.9 | 4.8 | 2.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Acetylcholinesterase/ACHE into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 82% to 120% with an overall mean recovery of 99%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 9.04-19.15 | 100 | 12.18 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: Acetylcholinesterase/ACHE
Acetylcholinesterase (AChE) is an enzyme located in the postsynaptic membrane and in the muscle endplates, where it hydrolyses the neurotransmitter acetylcholin. AChE from brain is a tetramer (G4-AChE) with a molecular mass of 320 kDa, AChE from erythrocytes is a dimer (G2-AChE) with a molecular mass of 170 kDa.
