Human ACE2 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.38 pg/ml (50 μl)；34.73 pg/ml (10 μl)； |
| Standard Curve Range | 13.72~10000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 10 μl/50 μl |
| Type | Ready-to-Use |
| Operation Duration | 120 min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0210 | 0.0218 | 0.0214 | |
| 13.72 | 0.0388 | 0.0313 | 0.0351 | 0.0137 |
| 41.15 | 0.0556 | 0.0485 | 0.0521 | 0.0307 |
| 123.46 | 0.1085 | 0.1030 | 0.1058 | 0.0844 |
| 370.37 | 0.2724 | 0.2717 | 0.2721 | 0.2507 |
| 1111.11 | 0.7754 | 0.7572 | 0.7663 | 0.7449 |
| 3333.33 | 1.8560 | 1.8550 | 1.8555 | 1.8341 |
| 10000.00 | 3.5960 | 3.6320 | 3.6140 | 3.5926 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 150.6 | 785.4 | 2461.7 | 149.6 | 799.6 | 2674.8 |
| Standard Deviation | 7.0 | 21.8 | 103.1 | 7.3 | 14.8 | 147.9 |
| Coefficient of Variation(%) | 4.7 | 2.8 | 4.2 | 4.9 | 1.9 | 5.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human ACE2 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 78% to 124% with an overall mean recovery of 83%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 2.34-296.61 | 100 | 139.11 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of ACE2 in this assay. No medical histories were available for the donors.
n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: ACE2
ACE-2, also called ACEH (ACE homolog), is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE. Human ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of human somatic ACE. The predicted human ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 mRNA is found at high levels in testis, kidney, and heart and at moderate levels in colon, small intestine, and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity. Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo.
ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2.
