Human DKK1 ELISA Kit检测试剂盒
| 规格 | 96 Test |
|---|---|
| 灵敏度 | 1.37 pg/ml (50 μl)；10.35 pg/ml (10 μl)； |
| 标准曲线范围 | 13.72~10000 pg/ml |
| 标准曲线梯度 | 7点/3倍 |
| 孵育次数 | 2 |
| 可检测样本 | 可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。 |
| 加样体积 | 50 μl/10 μl |
| 类型 | 完全即用型 |
| 操作时长 | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0096 | 0.0093 | 0.0095 | |
| 13.72 | 0.0204 | 0.0206 | 0.0205 | 0.0111 |
| 41.15 | 0.0493 | 0.0476 | 0.0485 | 0.0390 |
| 123.46 | 0.1434 | 0.1343 | 0.1389 | 0.1294 |
| 370.37 | 0.4189 | 0.4093 | 0.4141 | 0.4047 |
| 1111.11 | 1.1580 | 1.1070 | 1.1325 | 1.1231 |
| 3333.33 | 2.5370 | 2.4540 | 2.4955 | 2.4861 |
| 10000.00 | 3.7700 | 3.7340 | 3.7520 | 3.7426 |
精度
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 214.0 | 991.5 | 3116.4 | 191.8 | 950.2 | 3209.4 |
| Standard Deviation | 7.2 | 33.5 | 130.7 | 5.3 | 23.7 | 122.8 |
| Coefficient of Variation(%) | 3.3 | 3.4 | 4.2 | 2.8 | 2.5 | 3.8 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 22 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
加标回收
健康人血清加入3个不同浓度水平的人DKK1，未加入人DKK1的血清作为本底，计算回收率。
回收率的范围从105%至120%，平均回收率为113%。
样本值
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 2716.70-5354.17 | 100 | 3894.92 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
产品数据表
背景: DKK1
Dickkopf 相关蛋白 1 (Dkk-1) 是 Dickkopf 蛋白家族的创始成员，该家族包括 Dkk-1、-2、-3、-4 和相关蛋白 Soggy。 Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region.每个结构域包含十个半胱氨酸残基。成熟的人 Dkk-1 是一种 40 kDa 糖基化蛋白，与小鼠、大鼠、兔和牛 Dkk-1 分别具有 86%、87%、90% 和 91% 的 aa 串行同一性。 It also shares 42% and 36% aa identity with human Dkk-2 and Dkk-4, respectively. Dkk-1 and Dkk-4 are well documented antagonists of the canonical Wnt signaling pathway.该途径通过受体复合物的 Wnt 接合而激活，该受体复合物由 Frizzled 蛋白和两种低密度脂蛋白受体相关蛋白 LRP5 或 LRP6 之一组成。 Dkk-1 antagonizes Wnt by forming ternary complexes of LRP5/6 with Kremen1 or Kremen2. Dkk‑1/LRP6/Krm2 complex internalization has been shown to down-regulate Wnt signaling. Dkk-1 is expressed throughout development and antagonizes Wnt-7a during limb development. Other sites of expression include developing neurons, hair follicles and the retina of the eye. The balance between Wnt signaling and Dkk-1 inhibition is critical for bone formation and homeostasis. Insufficient or excess Dkk-1 activity in bone results in increased or decreased bone density, respectively. In adults, Dkk-1 is expressed in osteoblasts and osteocytes, and neurons. Cerebral ischemia induces Dkk-1 expression, which contributes to neuronal cell death.
